1978年美国Sensabaugh分离出精浆特异蛋白P30,并成功的制备出相应的抗P30血清。1987年我国也研制出抗人精浆特异蛋白P30血清,并应用该种抗血清建立了几种琼脂扩散和电泳方法鉴定人精斑。由于人精液中P30含量不高,平均为1.52±0.676mg/ml,鉴定微量精斑存在困难。为了更有效的提高方法的灵敏度。我们建立了鉴定微量人精斑的ELISA固相P30抗体法,现报告如下。 Sensabaugh in 1978 the United States isolated seminal plasma specific protein P30, and the successful preparation of the corresponding anti-P30 serum. In 1987 China also developed anti-human serum-specific protein P30 serum, and the application of this antiserum established several agar diffusion and electrophoresis identification of human semen. As the human semen P30 content is not high, with an average of 1.52 ± 0.676mg / ml, identification of trace semen difficulties. In order to more effectively improve the sensitivity of the method. We have established the identification of trace human semen ELISA solid phase P30 antibody method, are as follows.