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精子在附睾内的运行过程中，结构和功能发生变化，最终达到成熟，并贮存于附睾尾部。本文用５－Ｄｏｘｙｌ－ｓｔｅａｒｉｃａｃｉｄ（５ＤＳＡ）和１６－Ｄｏｘｙｌ－ｓｔｅａｒｉｃａｃｉｄ（１６ＤＳＡ）为标记，在电子自旋共振（ＥＳＲ）波谱仪上研究成年ＳＤ雄鼠附睾头、体、尾各段的精子膜流动性的变化，并用低渗膨胀（ＨＯＳ）试验测定精子膜对渗透压的反应能力。结果表明，从附睾头部到尾部，精子膜膜脂的流动性逐步降低，特别是从体部到尾部更为明显（Ｐ＜０．０１），而精子膜对渗透压的反应能力也逐渐降低，主要表现在从头部移行到体部的精子（Ｐ＜０．０１）。精子成熟过程中的脂质过氧化反应，可能是降低膜脂流动性的原因之一。
Sperm in the epididymis in the course of the operation, the structure and function changes, and ultimately reached maturity, and stored in the epididymis tail. In this study, sperm membrane flow in the head, tail and tail of adult male SD rats was studied by electron spin resonance (ESR) spectroscopy using 5-Doxyl-stearicacid (5DSA) and 16-Doxyl-stearicacid Sexual changes were measured and the ability of the sperm membrane to respond to the osmotic pressure was measured using the hypotonic swelling (HOS) test. The results showed that the fluidity of membrane lipids decreased gradually from the epididymis head to the tail, especially from the body to the tail (P <0.01), while the sperm membrane’s ability to respond to the osmotic pressure gradually decreased , Mainly in sperm migrating from the head to the body (P <0.01). Lipid peroxidation during sperm maturation may be one of the reasons to reduce the fluidity of membrane lipids.