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目的探讨水飞蓟宾对快速老化小鼠(SAMP8)学习记忆及氧化应激因子表达影响。方法以SAM P8小鼠为模型,采用新物体辨别、Y迷宫及M orris水迷宫进行学习记忆能力评价,酶联免疫实验检测氧化应激相关指标。结果与对照组比较,模型组小鼠优先指数(0.45±0.07)、自发交替反应率[(0.58±0.06)%]明显下降,逃避潜伏期[(50.62±11.24)s]明显延长(P<0.01),小鼠海马组织中谷胱甘肽(GSH)、超氧化物歧化酶(SOD)水平[分别为(21.13±6.17)mg/mL、(15.21±0.09)μg/mL]降低,丙二醛含量[(4.02±0.12)nmol/mL]升高(P<0.05);与模型组比较,水飞蓟宾200 mg/kg组小鼠优先指数(0.68±0.09)、自发交替反应率[(0.70±0.09)%]升高,逃避潜伏期[(23.45±11.94)s]明显缩短(P<0.01),小鼠海马组织中GSH、SOD水平[分别为(42.37±12.08)mg/mL、(32.24±0.24)μg/mL]升高,丙二醛含量[(2.54±0.21)nmol/mL]明显降低(P<0.05)。结论水飞蓟宾可改善快速老化小鼠学习记忆障碍,其机制可能与提高小鼠抗氧化应激能力有关。
Objective To investigate the effect of silybin on learning and memory and oxidative stress factor (srf) expression in rapidly aging mice (SAMP8). Methods SAM P8 mice were used as model to evaluate the learning and memory abilities by new object discrimination, Y maze and Mris water maze. Enzyme - linked immunosorbent assay was used to detect the indexes related to oxidative stress. Results Compared with the control group, the model group showed a significant decrease in the priority index (0.45 ± 0.07) and spontaneous alternation rate [(0.58 ± 0.06)%], and the escape latency [(50.62 ± 11.24) s] , The levels of glutathione (GSH) and superoxide dismutase (SOD) in the hippocampus of mice [(21.13 ± 6.17 mg / mL, (15.21 ± 0.09) μg / mL, (4.02 ± 0.12) nmol / mL] (P <0.05). Compared with the model group, the preference index (0.68 ± 0.09) and the spontaneous alternation rate [(0.70 ± 0.09) (42.37 ± 12.08) mg / mL and (32.24 ± 0.24), respectively, in the hippocampus of mice [(23.45 ± 11.94) s] μg / mL], MDA content [(2.54 ± 0.21) nmol / mL] was significantly decreased (P <0.05). Conclusion Silybin can improve the learning and memory impairment in rapidly aged mice, and its mechanism may be related to its ability of enhancing anti-oxidative stress in mice.