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目的 探讨膀胱肿瘤的基因治疗途径。方法 利用Lipofectamine将外源性野生型p53基因导入人膀胱移行细胞癌TBC 1细胞中 ,并得到表达 ,检测多项生物学指标。结果 被转染的细胞在体外标准条件下 ,生长抑制率为 56 .52 % ;细胞周期分析显示G0 +G1 细胞比例由 38.60 %增高到 52 .60 % ,凋亡指数 1 0 .1 6 %升高到 47.2 8% (P <0 .0 1 ) ;细胞生长速度减低 ;代表细胞增殖能力AgNORs计数从 6 .1 9下降为 4 .33(P <0 .0 1 ) ;对丝裂霉素和阿霉素的敏感性提高。结论 增加膀胱肿瘤细胞内野生型 p53的基因表达能抑制恶性肿瘤细胞的生长 ,提高肿瘤细胞对化疗药的敏感性。
Objective To investigate the gene therapy approach of bladder tumor. Methods The exogenous wild-type p53 gene was transfected into human bladder transitional cell carcinoma TBC 1 cells by Lipofectamine, and expressed and detected a number of biological indicators. Results The growth inhibition rate of the transfected cells was 56.52% under the standard conditions in vitro. The cell cycle analysis showed that the proportion of G0 + G1 cells increased from 38.60% to 52.60%, and the apoptotic index was 120.1% Up to 47.2 8% (P <0.01); the cell growth rate decreased; the proliferation index of AgNORs decreased from 6.19 to 4.33 (P <0.01) Adriamycin increased sensitivity. Conclusion Increasing the expression of wild-type p53 in bladder tumor cells can inhibit the growth of malignant tumor cells and increase the sensitivity of tumor cells to chemotherapeutic drugs.