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以“赤霞珠”葡萄为试材,对组培快繁中的取材部位、消毒方法、不同激素用量、是否带叶继代培养等关键技术措施进行了研究探讨。结果表明:以半木质化,0.1%升汞溶液消毒8min的茎段接种,成活率高,污染率低;并且继代培养和生根培养最好的培养基为1/2MS+IBA0.2mg·L~(-1)+蔗糖20g·L~(-1)+琼脂7g·L~(-1)。
Taking “Cabernet Sauvignon” grapes as test material, the key technical measures such as the site of tissue culture, the method of disinfection, the dosage of different hormones, whether the leaves were subcultured were studied. The results showed that the half-lignified, 0.1% mercuric chloride solution disinfected 8min inoculation of stem segments, high survival rate, low contamination rate; and subculture and rooting culture medium best 1 / 2MS + IBA0.2mg · L ~ (-1) + sucrose 20g · L ~ (-1) + agar 7g · L ~ (-1).