We systematically investigate dynamic separation of Xe and Kr at room temperature using four representative porous materials(Cu-BTC, ZIF-8, COP-4 and activated carbon(AC)). Results indicate that among the four materials, Cu-BTC not only shows the highest retention volume per gram(V_g=788 m L g~(-1), which is 1.8 times of activated carbon(436 m L g~(-1))) under flowing condition, but also can separate 350 ppm Xe from 35 ppm Kr mixture in air with a high Xe/Kr selectivity of 8.6 at room temperature and 200 k Pa, due to its suitable pore morphology, open metal sites, small side pockets in the framework. Moreover, the Cu-BTC also performs well on individual separation of Xe, Kr, CO_2 from five-component gas mixture(Xe:Kr:CO_2:Ar:N_2= 1:1:1:1:0.5, V/V) and has the longest retention time for Xe(20 min) in gas chromatographic separation, suggesting that it is a good candidate for potential applications as polymeric sieves. We systematically investigate dynamic separation of Xe and Kr at room temperature using four representative porous materials (Cu-BTC, ZIF-8, COP-4 and activated carbon (AC) shows the highest retention volume per gram (V_g = 788 m L g -1, which is 1.8 times of activated carbon (436 m L g -1)) under flowing condition, but also can separate 350 ppm Xe From 35 ppm Kr in air with a high Xe / Kr selectivity of 8.6 at room temperature and 200 k Pa, due to its suitable pore morphology, open metal sites, small side pockets in the framework. well on individual separation of Xe, Kr, CO2 from the five-component gas mixture (Xe: Kr: CO2: Ar: N2 = 1: 1: 1: 1: 0.5, V / V) and has the longest retention time for Xe 20 min) in gas chromatographic separation, suggesting that it is a good candidate for potential applications as polymeric sieves.