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AIM:To examine the protein expression alterations in liverinjury/repair network regulation as a response to gut-derivedlipopolysaccharide (LPS) treatment,in order to anticipatethe possible signal molecules or biomarkers in signaling LPS-related liver injury.METHODS:Male BALB/c mice were treated with intra-peritoneal (i.p.) LPS (4 mg/kg) and sacrificed at 0,6,24and 30 h to obtain livers.The livers were stained withhematoxylin and eosin for histopathologic analyses.Totalliver protein was separated by two-dimensional gelelectrophoresis (2-DE).The peptide mass of liver injury orrepair related proteins were drawn up and the proteindatabase was searched to identify the proteins.RESULTS:Observations were as follows:(1) TRAIL-R2was down regulated in livers of LPS-treated mice.TNFAIP1was significantly up regulated at 6 h,then down- regulatedat 24,30 h with silent expression during senescent stage.(2) The amount of metaxin 2 and mitochondria import innermembrane translocase subunit TIN8a (TINMSA) wasincreased upon treatment with LPS.(3) P34 cdc2 kinasewas significantly up-regulated 30 h after LPS administrationwith silent expression during senescent,6,24 h treatedstage.(4) The amount of proteasome activator 28 alphasubunit (PA28),magnesium dependent protein phosphatase(MDPP) and lysophospholipase 2 was decreased 6 h afterLPS treatment but recovered or up-regulated 24 and 30 hafter LPS treatment.CONCLUSION:LPS-treated mouse liver displaying a time-dependent liver injury can result in expression change ofsome liver injury or repair related proteins.
AIM: To examine the protein expression alterations in liverinjury / repair network regulation as a response to gut-derived lipopolysaccharide (LPS) treatment, in order to anticipate the possible signal molecules or biomarkers in signaling LPS-related liver injury. METHODS: Male BALB / c mice were treated with intra-peritoneal (ip) LPS (4 mg / kg) and sacrificed at 0,6,24 and 30 h to obtain livers. The livers were stained with hematoxylin and eosin for histopathology analyzes. Totalliver protein was separated by two-dimensional gelelectrophoresis (2-DE) .The peptide mass of liver injury orrepair related proteins were drawn up and the proteindatabase was searched to identify the proteins.RESULTS: Observations were as follows: (1) TRAIL-R2was down regulated in livers of LPS-treated mice (2) The amount of metaxin 2 and mitochondria import innermembrane translocase subunit TIN8a (TINMSA (4) The amount of proteasome activator 28 alphasubunit (PA28), magnesium dependent protein (PA28), was significantly increased in the LPS-treated and LPS- LPS-treated mouse liver displaying a time-dependent liver injury can result in expression change ofsome liver injury or repair (MDPP) and lysophospholipase 2 was decreased 6 h after LPS treatment or recovered or up-regulated 24 and 30 hafter LPS treatment. related proteins.