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目的 :用基因芯片研究 PMA激活的血管内皮细胞的早期反应基因 (imm ediate early response gene,ERG)。方法 :以包含 40 96条人类基因的 DNA芯片检测血管内皮细胞受代谢增强剂 PMA(phorbol myristate acetate)激活后早期的基因表达谱 ,并从中筛查出 ERG。结果 :血管内皮细胞受 PMA作用 6 h后 ,17条基因上调 ,11条下调。数据处理聚类分析表明 17条上调基因中多数 (13/ 17)属蛋白质磷酸酶和转录调控因子基因 ,而下调基因中多数 (9/ 11)为细胞分裂相关基因。结论 :证明PMA激活的人血管内皮细胞早期反应基因主要是转录调控因子和蛋白质磷酸酶基因。
OBJECTIVE: To study the imm ediate early response gene (ERG) of PMA-activated vascular endothelial cells by microarray. Methods: The DNA microarray containing 40 96 human genes was used to detect the early gene expression profiles of vascular endothelial cells activated by phorbol myristate acetate (PMA) and the ERG was screened out. Results: After 6 h of PMA treatment, 17 genes were up-regulated and 11 down-regulated. Data processing cluster analysis showed that most of the 17 up-regulated genes (13/17) were protein phosphatase and transcriptional regulator genes, while the majority of down-regulated genes (9/11) were cell-division related genes. CONCLUSION: The PMA activated human vascular endothelial cells early response genes are mainly transcription regulatory factor and protein phosphatase gene.