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适合番茄叶肉原生质体培养的材料是３～４周龄无菌苗顶端开展的叶片，在２７℃下，采用１０ｇ／Ｌ纤维素酶“Ｏｎｏｚａｋａ”Ｒ－１０、５ｇ／Ｌ果胶酶Ｐｅｃｔｉｎａｓｅ、５ｍｍｏｌ／ＬＭＥＳ、９０ｇ／Ｌ甘露糖醇、ＣＰＷ盐的酶液游离撕去下表皮的嫩叶，１４～１６ｈ，效果最好。研究表明，“红玫瑰”番茄和Ｌ．ｅｓｃ．×（Ｌ．ｅｓｃ．×Ｓ．ｌｙ．，Ｆ１），ＢＣ１Ｆ１的酶解效果最好，原生质体的产量和活力都最高；秘鲁番茄和Ｌ．ｅｓｃ．×Ｓ．ｌｙ．，Ｆ１次之；而潘那利番茄酶解效果最差，产量依次为：４８３×１０６、４５５×１０６、４３７×１０５、２１２×１０５和３０３×１０４个／ｇ。试验中，对多种培养基及培养方法进行对比研究，结果表明，Ｄ２培养基液体浅层法培养效果最好，ＴＭ－２液体浅层培养和Ｍ８Ｅ琼脂包埋漂浮法次之。在所采用的５种试材中，从“红玫瑰”番茄和Ｌ．ｅｓｃ．×（Ｌ．ｅｓｃ．×Ｓ．ｌｙ．，Ｆ１），ＢＣ１Ｆ１的叶肉原生质体获得了愈伤组织。
The material suitable for protoplast culture of tomato mesophyll was the top of the leaves of 3 to 4 weeks old sterile seedlings. At 27 ℃, 10 g / L cellulase “Onozaka” R-10, 5 g / L pectinase Pectinase, / LMES, 90g / L of mannitol, CPW salt of the enzyme solution to remove the lower epidermal leaves, 14 ~ 16h, the best. Research shows that “red rose” tomatoes and L. esc. × (L.esc. × S.ly., F1). BC1F1 had the best enzymolysis effect and the highest protoplast yield and vigor. esc. × S. ly. , Followed by F1; Panzhihua tomato hydrolyzate the worst, the yield as follows: 4 83 × 106,4 55 × 106,4 37 × 105,2 12 × 105 and 3 × 103 × 104 / G. In the experiment, a comparative study on various media and culture methods was carried out. The results showed that liquid culture of D2 medium was the best, and TM-2 liquid shallow culture followed by M8E agar embedding and floating method. Among the five kinds of test materials used, “Red Rose” tomato and L. esc. × (L.esc. × S.ly., F1). Callus was obtained from mesophyll protoplasts of BC1F1.