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目的:观察大鼠缺血性脑损伤对大、小脑波谱的影响以及当归对大鼠缺血性脑损伤代谢物质的变化。方法:18只雄性Wistar大鼠,体重150—200g,随机分成正常对照组(n=6)、缺血组(n=6)、当归治疗组(n=6)。采用大脑中动脉阻断法(middlecerebralarteryocclusion,MCAO)制作大鼠急性脑缺血损伤模型,缺血3h后,恢复血供或当归治疗,采用离体高分辨魔角旋转波谱(1HHRMASNMR)技术检测,观察大、小脑波谱中胆碱(Cho)、肌酸(Cre)、N-乙酰天门冬氨酸(NAA)、γ-氨基丁酸(GABA)、谷氨酸(Glu)、天冬氨酸(Asp)、甘氨酸(Gly)等共振峰的变化。结果:除Cho,Glu,Asp升高,大鼠急性脑缺血时大多数代谢物含量均明显低于正常对照组,经当归治疗后其代谢物的含量会有所增加。结论:缺血严重影响大、小脑神经元的存活和物质代谢,当归对大鼠缺血性脑损伤具有明显的保护作用。
Objective: To observe the effect of ischemic brain damage on the spectrum of large and small cerebellum and the changes of metabolic substances in ischemic brain damage in rats. Methods: Eighteen male Wistar rats weighing 150-200 g were randomly divided into normal control group (n=6), ischemic group (n=6), and angelica treatment group (n=6). Acute cerebral ischemic injury in rats was induced by middle cerebral artery occlusion (MCAO). After 3 hours of ischemia, blood supply or angelica treatment was resumed. In vitro high-resolution magic angle rotation spectroscopy (1HHRMAS NMR) was used to observe the lesions. In the cerebellar spectrum, choline (Cho), creatine (Cre), N-acetylaspartic acid (NAA), γ-aminobutyric acid (GABA), glutamic acid (Glu), aspartic acid (Asp) Changes in the resonance peaks such as glycine (Gly). RESULTS: Except for Cho, Glu, and Asp, most of the metabolites in rats with acute cerebral ischemia were significantly lower than those in the normal control group. The content of metabolites increased after Angelica sinensis treatment. Conclusion: Ischemia severely affects the survival and metabolism of large and small cerebellar neurons. Angelica has a protective effect on ischemic brain injury in rats.