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[目的]探讨携带内皮抑素基因腺病毒对卵巢癌HO-8910细胞的抑制作用。[方法]采用细胞培养、光镜、电镜、MTT法、RT-PCR、Western blot、TUNEL法分析检测结果。[结果]重组腺病毒pLP-Ad-ES能够引起HO-8910细胞凋亡细胞形态结构改变;pLP-Ad-ES能够抑制卵巢癌细胞HO-8910的增殖(P<0.01),并且能够促进细胞凋亡,存在时间—剂量依赖关系;在基因蛋白水平上,随着滴度的增加,内皮抑素的表达增加。[结论]pLP-Ad-ES对卵巢癌HO-8910细胞有明显的抑制作用,能够诱导细胞凋亡。
[Objective] To investigate the inhibitory effect of endostatin gene-carrying adenovirus on HO-8910 ovarian cancer cells. [Method] The test results were analyzed by cell culture, light microscope, electron microscope, MTT method, RT-PCR, Western blot and TUNEL. [Results] The recombinant adenovirus pLP-Ad-ES could induce morphological changes of apoptotic cells in HO-8910 cells. PLP-Ad-ES could inhibit the proliferation of ovarian cancer cells HO-8910 (P <0.01) There was a time-dose-dependent relationship. At the level of gene protein, the expression of endostatin increased with the increase of titer. [Conclusion] pLP-Ad-ES can obviously inhibit HO-8910 ovarian cancer cells and induce apoptosis.