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目的为研究者选择合适的牛磺胆酸钠浓度来制备大鼠重症胰腺炎(severe acute pancreatitis,SAP)SAP模型提供依据。方法将60只SD大鼠随机分为假手术组、1.5%浓度组、3.5%浓度组和5%浓度组,造模各组分别用1.5%、3.5%和5%牛磺胆酸钠按逆行胆胰管注射法制备SAP模型。术后统计各组大鼠的死亡率;检测血清淀粉酶、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平;观测各组大鼠胰腺组织HE染色病理评分。结果5%浓度组死亡率较1.5%浓度组显著升高,血淀粉酶、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平、出血和腺泡组织坏死的病理评分较1.5%浓度组和3.5%浓度组均有显著升高。结论 5%的牛磺胆酸钠逆行胆胰管注射法能更好的制备SAP模型,且更符合SAP的生理、病理表现。
Objective To provide a basis for the researchers to choose the appropriate concentration of sodium taurocholate to prepare the rat SAP model of severe acute pancreatitis (SAP). Methods Sixty Sprague-Dawley rats were randomly divided into sham operation group, 1.5% concentration group, 3.5% concentration group and 5% concentration group. The rats in each model were treated with 1.5%, 3.5% and 5% Preparation of SAP model by biliary and pancreatic duct injection. The mortality of rats in each group was calculated. The levels of serum amylase, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured and the pathological scores of pancreas were observed. Results The mortality rate in 5% concentration group was significantly higher than that in 1.5% concentration group. The levels of serum amylase, tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), hemorrhage and acinar necrosis Compared with 1.5% concentration group and 3.5% concentration group were significantly increased. Conclusion 5% sodium taurocholate retrograde cholangiopancreatography can better prepare SAP model, and more in line with the physiological and pathological SAP.