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目的:探讨miR-1/133基因簇(miR-1/133cluster)在肝癌组织中的表达、与临床表型的相关性及miR-1对肝癌细胞HepG2增殖与凋亡的影响。方法:检测38例肝癌及相应癌旁组织中miR-1及miR-133b的表达,并分析表达水平与临床表型的相关性。分别将miR-1-3p模拟物(miR-1-3p mimics)、模拟物阴性对照(mimics NC)转染至HepG2,CCK-8法、流式细胞术分别检测细胞增殖、活力与凋亡水平。结果:miR-1-3p、miR-133b在肝癌组织中较癌旁组织降低(P=0.001,0.028)。miR-1-3p、miR-133b与肝癌TNM分期负相关(P=0.016,0.027),miR-133b与肝癌肿瘤个数负相关(P=0.001)。miR-1-3p mimics转染至HepG2细胞,较NC组的细胞增殖降低、细胞凋亡增加,且细胞周期阻滞于S期。结论:肝癌低表达的miR-1-3p及miR-133b与肝癌分期、肿瘤个数负相关,miR-1抑制肝癌细胞增殖并促进细胞凋亡,miR-1/133基因簇在肝癌中发挥抑癌作用。
Objective: To investigate the expression of miR-1 / 133cluster in hepatocellular carcinoma and its relationship with clinical phenotype and the effect of miR-1 on the proliferation and apoptosis of HepG2 hepatocellular carcinoma cells. Methods: The expression of miR-1 and miR-133b in 38 cases of HCC and corresponding paracancerous tissues was detected, and the correlation between the expression level and clinical phenotype was analyzed. The miR-1-3p mimics and mimics NC were transfected into HepG2 and CCK-8 respectively, and the cell proliferation, viability and apoptosis were detected by flow cytometry . Results: miR-1-3p and miR-133b were lower in HCC tissues than those in paracancer tissues (P = 0.001,0.028). miR-1-3p and miR-133b were negatively correlated with TNM staging of HCC (P = 0.016,0.027), miR-133b was negatively correlated with the number of HCC (P = 0.001). miR-1-3p mimics transfected HepG2 cells, compared with NC cell proliferation decreased, increased apoptosis, and cell cycle arrest in the S phase. Conclusions: The low expression of miR-1-3p and miR-133b in hepatocellular carcinoma is negatively correlated with the staging of tumor and the number of tumor cells. MiR-1 inhibits the proliferation of hepatocellular carcinoma cells and promotes the apoptosis of tumor cells. Cancer effect.