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目的:研究朱砂七总蒽醌(ZSQ)对HL-60细胞增殖抑制和诱导凋亡的作用,探讨其抗肿瘤作用及其机制。方法:应用MTT法检测朱砂七总蒽醌对HL-60细胞的生长抑制作用,Giemsa染色观察细胞形态学改变,DNA ladder和流式细胞术等方法检测朱砂七总蒽醌诱导肿瘤细胞凋亡及对细胞周期的作用。结果:32、3.2、0.32、0.032mg/ml朱砂七总蒽醌作用HL-60细胞72h后,细胞生长抑制率分别为28.69%,36.84%,63.76%和80.57%,抑制作用呈时间及浓度依赖性,朱砂七总蒽醌在48h的IC50约为3.2mg/ml。形态学观察到用药后凋亡细胞典型的形态学特征,伴有多核细胞形成。FCM分析发现朱砂七总蒽醌阻断HL-60细胞生长于细胞周期的G2/M期,诱导凋亡作用呈时间及浓度依赖性,能够诱发肿瘤细胞产生凋亡小体、DNA ladder和细胞凋亡峰。结论:朱砂七总蒽醌能抑制HL-60肿瘤细胞的增殖并诱导凋亡。
OBJECTIVE: To study the effect of total anthraquinone (ZSQ) on proliferation and apoptosis of HL-60 cells and its anti-tumor effect and its mechanism. Methods: MTT assay was used to detect the growth inhibition effect of total anthraquinone on HL-60 cells. Giemsa staining was used to observe the morphological changes of cells. DNA ladder and flow cytometry were used to detect the apoptosis of tumor cells induced by total anthraquinone. The role of the cell cycle. Results: The cell growth inhibition rates of HL-60 cells treated with 32,3.2,0.32,0.032mg / ml cinnabar total anthraquinone for 72h were 28.69%, 36.84%, 63.76% and 80.57%, respectively. The inhibitory effects were time and concentration-dependent The IC50 of cinnabar total anthraquinone at 48h was about 3.2mg / ml. Morphology Apoptotic cells were observed after treatment typical morphological characteristics, accompanied by the formation of multi-nucleated cells. FCM analysis showed that total anthraquinone of cinnabar inhibited the growth of HL-60 cells in the G2 / M phase of the cell cycle, inducing apoptosis in a time and concentration-dependent manner, which could induce apoptosis of tumor cells, DNA ladder and apoptosis Peak. Conclusion: Total anthraquinone cinnabar inhibits the proliferation of HL-60 tumor cells and induces apoptosis.