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目的:观察扶肺煎对C57BL/6小鼠Lewis肺癌移植瘤的抑制作用,研究其对增殖细胞核抗原(PCNA)、血管内皮生长因子(VEGFA)及其受体(VEGFR-2)的影响。方法:70只雄性SPF级C57BL/6小鼠随机建立Lewis肺癌移植瘤模型,分别为空白对照组、模型对照组、扶肺煎低剂量组、扶肺煎中剂量组、扶肺煎高剂量组、顺铂组、扶肺煎中剂量联合顺铂组。每天观察动物一般情况及各组小鼠皮下结节情况并称重。第15天脱颈处死全部小鼠,完整剥离肿瘤组织并称重,计算各组抑瘤率及增效率,检测PCNA、VEGFA及VEGFR-2的表达。结果:扶肺煎联合顺铂组的抑瘤率最高,为65.81%。扶肺煎联合顺铂组增效率为107.91%,表明其对顺铂具有较好的增效作用。扶肺煎联合顺铂组PCNA阳性表达率低于其余各组,但差异无统计学意义(P>0.05)。扶肺煎联合顺铂组VEGFA、VEGFR-2的表达量均小于其余各组。结论:扶肺煎联合化疗可降低C57BL/6小鼠Lewis肺癌移植瘤中PCNA的表达并抑制肿瘤生长,降低Lewis肺癌移植瘤中VEGFA、VEGFR-2的表达,起到抑制肿瘤血管生成及增敏增效的作用。
Objective: To observe the inhibitory effect of Fufang Jian on Lewis lung carcinoma in C57BL / 6 mice and its effect on proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGFA) and its receptor (VEGFR-2). Methods: Forty Lewis male SPF C57BL / 6 mice were randomly divided into three groups: control group, model control group, Fufang decoction low dose group, Fufang decoction dose group, Fufang decoction high dose group , Cisplatin group, Fufang decoction combined with cisplatin group. The general situation of animals and the subcutaneous nodules in each group were observed daily and weighed. On day 15, all the mice were killed by atrophy and the tumor tissue was completely stripped and weighed. The inhibition rate and efficiency of each group were calculated, and the expressions of PCNA, VEGFA and VEGFR-2 were detected. Results: Fufei decoction combined cisplatin group had the highest inhibition rate of 65.81%. Fufang Jian combined cisplatin group increased efficiency of 107.91%, indicating that cisplatin has a good synergistic effect. The positive rate of PCNA expression in Fufang decoction plus cisplatin group was lower than that in other groups, but the difference was not statistically significant (P> 0.05). Fufei decoction combined with cisplatin group VEGFA, VEGFR-2 expression levels were less than the remaining groups. Conclusion: Fufang Jian combined with chemotherapy can reduce the expression of PCNA in Lewis lung carcinoma C57BL / 6 mice and inhibit tumor growth, reduce the expression of VEGFA and VEGFR-2 in transplanted Lewis lung carcinoma and inhibit tumor angiogenesis and sensitization The role of efficiency.