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美国夏威夷蔗农协会试验站在一九六一年最先用甘蔗的成熟薄壁组织来产生愈伤组织.接着又用这种愈伤组织进行悬浮培养,从中分离出几个单独的细胞,并使每一个细胞产生一个愈伤组织.所产生的愈伤组织的染色体数目,形态和生长特性都不一样,好像是几个不同的品系.后来发现供试品种是染色体的嵌合体.从而促使该站力图以此分离和培育出农艺性状有所改良的甘蔗新品种.在明确甘蔗愈伤组织的性能以后不久,就试图用细胞和组织的培养技术以分离出抗菲济病、霜霉病品系和嵌纹病的亚系.
The Hawaiian Sugarcane Experimental Station of the United States first used callus from mature parenchyma of sugarcane to produce callus in 1961. The callus was then used for suspension culture and several individual cells were isolated from Each cell produces a callus, and the resulting callus has a different number of chromosomes, morphological and growth characteristics, as if it were several different lines, and later found to be a chimera of the chromosome, prompting the station Trying to isolate and cultivate new varieties of sugarcane with improved agronomic traits.After identifying the properties of sugarcane callus, we tried to use cell and tissue culture techniques to isolate anti-Fiji and downy mildew strains and Subline of the mosaic disease.