本文研究了应用薄层层析一比色法测定活络镇风丸及马钱子中士的宁的含量。样品提取液在硅胶G薄层上,用丙酮∶氯仿∶浓氨水(30∶15∶1)为展开剂,稀碘化铋钾试液显色定位。刮下士的宁斑点,洗脱,用0.1%溴麝香草酚兰溶液处理后,加0.01N氢氧化钾无水乙醇溶液10分钟显色,在610nm处测定吸收度。本法简便、灵敏、稳定。 This paper studied the application of TLC-colorimetry to determine the content of Shiroinzhen Fengfeng Pill and Strychnos nucifera. The sample extract was applied on a thin layer of silica gel G with acetone:chloroform:concentrated aqueous ammonia (30:15:1) as developing solvent, and the cesium-potassium dilute iodide test solution was colorimetrically located. The strychnine spot was scraped off, eluted, treated with a 0.1% solution of bromothymol blue, added with a 0.01 N potassium hydroxide anhydrous ethanol solution for 10 minutes, and the absorbance was measured at 610 nm. This method is simple, sensitive and stable.