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目的:了解福建省三明沙县医院健康人群输血传播病毒(TTV)的感染情况方法:准备212名健康人群健康人群者血浆。使用UTR和G1-G5群特异性巢式PCR,检测血浆中TTV的UTR-DNA以及5种基因群的DNA。将1-5群部分PCR阳性产物(每1群各两份)进行核酸序列测定,并与15种已定群别的TTV代表株进行同源性比较分析。统计学方法:使用卡方检验(X~2)对检测结果进行分析。结果:(1)使用改良UTR-PCR方法测定,健康人群者TTV感染率为95.3%(212/212),(2)使用群特异性PCR检测TTV,G4群TTV感染率最高,为52.0%(105/212),然后依次为G5:39.1%(79/212),G3:32.7%(66/212),G1:25.7%(52/212),G2:8.4%(17/212)。)。结论:TTV易发生混合群感染,即同一个实验对象可以检测出不同种属于不同群TTV感染。部分阳性产物所测的核酸序列与TTV病毒代表株的核酸序列进行比较分析,认为TTV分群PCR测定是一种可行的TTV感染率检测手段。
Objective: To understand the infection status of blood transfusion transmitted virus (TTV) among healthy population in Sanming Sha County Hospital of Fujian Province Method: Prepare 212 healthy human plasma. Using UTR and G1-G5 population-specific nested PCR, UTR-DNA of plasma TTV and DNA of five gene clusters were detected. A total of 1-5 groups of PCR-positive products (two for each population) were subjected to nucleic acid sequencing and comparative analysis of homology with 15 representative TTV representative strains. Statistical Methods: The test results were analyzed using the chi-square test (X ~ 2). Results: (1) TTV infection rate in healthy population was 95.3% (212/212) using modified UTR-PCR method. (2) TTV infection was detected by group specific PCR, the highest infection rate was 52.0% Followed by G5: 39.1% (79/212), G3: 32.7% (66/212), G1: 25.7% (52/212), G2: 8.4% (17/212). ). Conclusion: TTV is prone to mixed group infection, that is, the same experimental subject can detect different groups belonging to different groups of TTV infection. Some positive products of nucleic acid sequences and TTV virus representative nucleic acid sequence comparison analysis, that TTV clustered PCR assay is a viable means of TTV infection detection.