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采用磷脂脂肪酸方法和Biolog-ECO微平板检测法,以吉林省长春市双阳区农业技术推广站实验区的土壤为对象,研究了水稻种植对黑土微生物生态结构和功能的影响.结果表明空地中有机质含量最高,其次为田间土,根际土中有机质含量最低;在夏季的样品中有机质含量最高,土壤中全氮的变化趋势与土壤有机质比较相似.土壤微生物群落功能多样性指数则表现为夏季显著高于秋季和春季,春、秋两季差异不明显,冬季最低,空地中微生物群落的Shannon-Wiener指数和Pielou均匀度指数高于田间和根际.全部样品微生物生长步入稳定期时间和碳源的颜色平均变化率变化范围不同,时间分别为216、192、216、120 h,范围分别为0.52~0.84、0.82~1.28、0.40~0.84、0.05~0.48,表明春秋季微生物步入稳定期和对碳源的代谢量比较相似,夏季最大,冬季最小.研究结果为科学评价典型水稻种植黑土区土壤生境质量退化与恢复过程中微生物特征的变化提供参考.
Phospholipid fatty acid method and Biolog-ECO microtiter plate assay were used to study the effects of rice cultivation on the ecological structure and function of black soil microorganisms in the experimental area of Shuangyang District Agricultural Technology Extension Station in Changchun, Jilin Province.The results showed that the organic matter The content of soil organic matter was the highest in the soil, the lowest was in the soil in the field, the lowest in the rhizosphere soil, the highest in the summer, and the trend of total nitrogen in the soil was similar to that in the soil organic matter.The functional diversity index of soil microbial community was significant in summer There was no obvious difference between spring and autumn in autumn and spring, and the lowest in winter.The Shannon-Wiener index and Pielou evenness index of the open microbial community were higher than those in the field and the rhizosphere.The microbial growth of all samples reached the stable period and carbon The average color change rate of the source varied from 216,192,216,120 h, respectively, ranging from 0.52 to 0.84, from 0.82 to 1.28, from 0.40 to 0.84, and from 0.05 to 0.48, respectively, indicating that the microbial population entered a stable period in spring and autumn Metabolism of carbon source is relatively similar, the largest in summer, the smallest in winter.The results for the scientific evaluation of typical black soil cultivation of rice Characteristics of microbial quality deterioration restoration process a reference.