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目的 分析丙烯酰胺 (AA)诱导的NIH 3T3小鼠成纤维细胞微核 (MN)染色体组成 ,判断其遗传毒性。方法 用着丝粒和端粒DNA探针双色荧光原位杂交检测MN的染色体组成。结果 AA诱导的NIH 3T3小鼠成纤维细胞总MN率和既含着丝粒又含端粒信号的MN率均有显著的剂量 -反应关系 (r =0 .992 9、0 .9933,P <0 .0 1)。高剂量 (40 0 μg/ml)时 ,AA诱导的MN中 6 5 .9%既含着丝粒信号又含端粒信号 ;2 6 .0 %只含端粒信号 ;8.1%无任何杂交信号。AA诱导的含着丝粒信号MN率 (2 7.0× 10 -3 )是不含着丝粒信号MN率 (14.0× 10 -3 )的 1.6倍 ,差异有显著性(P <0 .0 1)。结论 AA既可诱导由染色体断片组成的MN ,又可诱导由整条染色体组成的MN。AA既是一种断裂剂又具有非整倍体毒性 ,高剂量时非整倍体毒性较明显。
Objective To analyze the chromosome composition of micronuclei (MN) of NIH 3T3 mouse fibroblasts induced by acrylamide (AA) and determine the genotoxicity. Methods Chromosome composition of MN was detected by two-color fluorescence in situ hybridization of centromere and telomere DNA probes. Results The total MN rate of NIH 3T3 mouse fibroblasts induced by AA was significantly dose-response relationship with the MN rates of both centromere and telomere (r = 0.992 9,0. 9933, P < 0 .0 1). At high doses (40 0 μg / ml), 6.59% of MN-induced MNs contained both centromere and telomere signals; 26.0% contained only telomere signals; and 8.1% did not have any hybridization signals . The MN induced centromere signal MN rate (2 7.0 × 10 -3) was 1.6 times that of centromere signal MN ratio (14.0 × 10 -3), the difference was significant (P <0.01) . Conclusions AA can induce both MN composed of chromosome fragments and MN composed of whole chromosomes. AA is both a cleavage agent and aneuploidy, aneuploidy is more toxic at high doses.