论文部分内容阅读
将培养至对数期的Ag8．653小鼠骨髓癌细胞经裂解、抽提、RnaseA处理和纯化，获得A260nm／A28nm，为2.013、电泳为一条区带的纯化的小鼠骨髓瘤细胞全DNA；该DNA经不同处理，标记两个探针，即全DNA通过缺口转移法，用‘中标记和全DNA用光敏生物素（军事医学科学院二所提供）标记?
Ag8.653 mouse bone marrow cancer cells cultured to the logarithmic phase were lysed, extracted, treated with RnaseA and purified to obtain A260nm / A28nm, which was 2.013. The purified DNA of mouse myeloma cells was electrophoresed into a band. The DNA is processed differently and two probes are labeled, ie, the whole DNA is nicked by the gap-transfer method and labeled with the ’middle’ marker and the whole DNA with the biotin (provided by the Military Academy of Medical Sciences)?