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Objective: The aim of our study was to investigate the relationship between cell apoptosis and dephosphorylatedRB protein and proliferating cell nuclear antigen (PCNA) in human breast cancer. Methods: MTT colorimetric assay was appliedto examine the growth inhibition, and the apoptosis was determined by flow cytometry (FCM). The expressing quantityof dephosphorylated RB protein and PCNA pre- and post the action of ADR were detected with immunocytochemistry. Results:MTT assay revealed that ADR inhibited proliferation of MCF-7/S cells in a dose dependent manner, the 50% inhibitionconcentration (IC50) value was 0.128 mg/L. Tumor cell apoptotic rate (AR) in ADR group (χ= 0.259) was significantly higherthan that in the control group (χ = 0.045) (P < 0.01). The expressive levels of dephosphorylated RB protein in ADR group(MOD × area = 986.8 ± 207.4) was significantly higher than that in control group (MOD × area =131.7 ± 31.9) (P < 0.01).PCNA positive expression rate in ADR group (χ = 0.3371) was significantly lower than that in the control group (χ = 0.5152)(P < 0.01). Conclusion: In ADR group, there was significant positive correlation between AR and the expressing quantity ofdephosphorylated RB protein, but there was significant negative correlation between AR and PCNA .