采用RT-PCR方法从小麦品种豫教2号发育的籽粒中克隆出淀粉合酶I基因(starch synthase I,SSI)部分cDNA片段(795 bp)(GenBank No.EF221762),同源性比较结果显示,它与GenBank上已报道的SSI基因有高度同源性。以p WM101质粒为基础,构建了由35S启动子调控的SS I基因的反义表达载体p WM101SSI;另外,还以pFGC5941质粒为基础,构建了SSI基因的RNAi载体pFGC5941SSIsa,这些载体的构建为研究此基因的功能奠定了基础。 A partial cDNA fragment (795 bp) of starch synthase I (SSI) was cloned from the wheat cultivar Yujiao No.2 by RT-PCR (GenBank No.EF221762). The homology comparison showed , Which has a high degree of homology with the reported SSI gene in GenBank. Based on p WM101 plasmid, an antisense expression vector pSW101SSI of SS I gene regulated by 35S promoter was constructed. In addition, an RNAi vector pFGC5941SSIsa of the SSI gene was constructed based on the pFGC5941 plasmid. The construction of these vectors was as follows: The function of this gene has laid the foundation.