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目的制作离体年轻恒牙早期龋模型,观察再矿化处理后釉质表面显微硬度及微观形貌,为学生恒牙龋病的防治提供理论依据。方法随机选取某大学学生离体年轻恒牙制备釉质样本60个,经酸蚀凝胶人工龋实验脱矿,建立早期龋模型,给予1.0%酪蛋白磷酸肽-磷酸钙(CPP-CP)再矿化液处理。比较各样本脱矿前后及再矿化后的显微硬度值,观察处理前后釉质表面微观形貌。结果脱矿前釉质表面显微硬度值为(252.53±18.31)KHV,脱矿后表面显微硬度明显下降到(168.76±16.99)KHV(t=2.053,P<0.05),再矿化后表面显微硬度提高到(216.17±15.48)KHV(t=2.047,P<0.05)。结论 CPP-CP再矿化处理对防治年轻恒牙早期龋有明显的临床意义。
Objective To study the early caries model of young permanent teeth in vitro and to observe the microhardness and micro-morphology of the enamel surface after remineralization, so as to provide a theoretical basis for the prevention and treatment of permanent caries in students. Methods A total of 60 enamel samples were prepared from young permanent teeth of a college student. Demineralization was performed on the acid-labile artificial dental caries to establish an early caries model. The caries were treated with 1.0% casein phosphate-calcium phosphate (CPP-CP) Liquid treatment. The microhardness values of the samples before and after demineralization and after remineralization were compared, and the morphology of enamel surface before and after treatment was observed. Results The microhardness of enamel surface before demineralization was (252.53 ± 18.31) KHV, the surface microhardness of the enamel surface after demineralization was significantly decreased to (168.76 ± 16.99) KHV (t = 2.053, P <0.05) Microhardness increased to (216.17 ± 15.48) KHV (t = 2.047, P <0.05). Conclusions CPP-CP remineralization has obvious clinical significance in preventing early caries of young permanent teeth.