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目的通过测定氧化乐果中毒Sprague-Dawley(SD)大鼠血清核转录因子-κB(NF-κB)的浓度、肺组织NF-κB表达水平、肺系数,探讨有机磷农药中毒致大鼠肺损伤的机制。方法将96只雄性健康SD大鼠(6周龄)随机分为对照组(A组)和染毒组(B组),每组48只,B组以0.4%氧化乐果乳油工作液(40%氧化乐果乳油1 mL加99 mL生理盐水稀释而成)以大鼠经口半数致死量45 mg/kg灌胃染毒建立有机磷中毒模型,A组灌胃等量无菌生理盐水。分别于灌胃后30 min及3、6、12、24、48 h从各组中抽取6只大鼠,并处死,测定各组肺系数、血清NF-κB浓度、肺组织NF-κB表达水平,并观察肺组织病理改变。结果 B组与A组比较,肺系数与血清NF-κB浓度及肺组织NF-κB表达上调(P<0.01);A组大鼠各时间点肺组织切片正常,B组大鼠肺组织于染毒后30 min出现肺间质充血,肺泡间隔增宽,甚至可见肺泡内有红细胞聚集,并逐渐加重,至染毒后12 h达到高峰,24~48 h上述变化逐渐减轻。结论 NF-κB可能参与了有机磷杀虫剂中毒时肺损伤的过程。
Objective To determine the expression of NF-κB, the expression of NF-κB and the pulmonary index in the rat model of Sprague-Dawley (SD) Mechanisms. Methods Ninety-six male healthy SD rats (6 weeks old) were randomly divided into control group (group A) and exposure group (group B), 48 rats in each group. Group B was treated with 0.4% omethoate EC % Omethoate EC 1 mL plus 99 mL of normal saline) was administered orally at a lethal dose of 45 mg / kg to establish a model of organophosphate poisoning. Group A was given the same volume of sterile saline. Six rats were randomly selected from each group at 30 min and 3, 6, 12, 24 and 48 h after gavage and sacrificed. The lung coefficient, serum NF-κB concentration and the expression of NF-κB , And observe the pathological changes of lung tissue. Results Compared with group A, the lung coefficient and the serum concentration of NF-κB and the level of NF-κB in lung tissue were increased in group B (P <0.01). The lung tissue sections of group A were normal at each time point, Toxoplasma hyperemia and widening of the alveolar septum occurred 30 min after poisoning. Even the accumulation of erythrocytes in the alveoli was observed and gradually increased. The peak was reached at 12 h after exposure and the above-mentioned changes gradually decreased from 24 to 48 h. Conclusion NF-κB may be involved in the process of lung injury induced by organophosphate insecticides.