[Objective]To establish the tissue culture system for budlets induced from the protocorm callus of Dendrobium candidum Wall. ex Lindl. ,and to optimize the hormone in culture medium. [Methods]Budlets were induced from the callus of D. candidum under three culture conditions in culture mediums with eight concentrations of 2,4-D. [Results] During callus induction,the optimal culture medium was 0. 5 mg/L 6-BA + 0. 5 mg/L NAA + (1. 5-2. 5) mg/L 2,4-D + 20. 0 g/L sucrose + 200. 0 g/L mashed potato + 2. 0 g/L activated carbon + 10. 0 g/L agar. And callus induction had the optimal effects under the culture condition of illumination after dark culture for 15 d. [Conclusions]The budlets were the maximum under the optimal culture condition mentioned above. [Objective] To establish the tissue culture system for budlets induced from the protocorm callus of Dendrobium candidum Wall. Ex Lindl., And to optimize the hormone in culture medium. [Methods] Budlets were induced from the callus of D. candidum under three culture conditions in culture mediums with eight concentrations of 2,4-D. [Results] During callus induction, the optimal culture medium was 0.5 mg / L 6-BA + 0.5 mg / L NAA + (1.5-2 . 5) mg / L 2,4-D + 20. 0 g / L sucrose + 200. 0 g / L mashed potato + 2.0 g / L activated carbon + 10. 0 g / L agar. And callus induction had the optimal effects under the culture condition of illumination after dark culture for 15 d. [Conclusions] The budlets were the maximum under the optimal culture condition mentioned above.