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目的通过观察埃兹蛋白(Ezrin)、金属蛋白酶-2(MMP-2)及金属蛋白细胞组织抑制因子-2(TIMP-2)蛋白水平在子宫颈癌组织中的表达情况,探讨它们与子宫颈癌侵袭转移的关系。方法采用免疫组织化学方法对40例子宫颈鳞癌,20例腺癌,29例子宫颈上皮内瘤样病变和16例正常子宫颈组织进行标记及分析。结果Ezrin、MMP-2及TIMP-2蛋白主要表达在子宫颈鳞癌、腺癌、上皮内瘤变的异型细胞质内,各实验组中的表达量与正常组比,差异均有统计学意义(P<0.01)。Ezrin和MMP-2蛋白在有淋巴结转移组中的表达量较无淋巴结转移组明显高(P<0.01),而TIMP-2蛋白水平的表达结果却相反。同时发现Ezrin、MMP-2及TIMP-2蛋白水平在宫颈病变组织表达有正相关性。结论Ezrin、MMP-2及TIMP-2蛋白在子宫颈鳞癌、腺癌的表达量较对照组高。Ezrin和MMP-2在有淋巴结转移的子宫颈癌中高表达,而TIMP-2为低表达,提示它们在子宫颈癌的侵袭转移中起重要的作用。Ezrin、MMP-2及TIMP-2蛋白水平在宫颈病变组织表达有正相关性,这三者在子宫颈癌的侵袭转移中可能起协同作用。
Objective To investigate the expression of Ezrin, metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) protein in cervical cancer and to explore their relationship with cervical Invasion and metastasis of cancer. Methods Immunohistochemistry was used to mark and analyze 40 cases of cervical squamous cell carcinoma, 20 cases of adenocarcinoma, 29 cases of cervical intraepithelial neoplasia and 16 cases of normal cervical tissue. Results Ezrin, MMP-2 and TIMP-2 were mainly expressed in the cytoplasm of cervical squamous cell carcinoma, adenocarcinoma and intraepithelial neoplasia. The expression levels of Ezrin, MMP-2 and TIMP-2 in each experimental group were significantly different from those in normal group P <0.01). The expression of Ezrin and MMP-2 protein in lymph node metastasis group was significantly higher than that in non-lymph node metastasis group (P <0.01), but the expression of TIMP-2 protein level was opposite. Also found that Ezrin, MMP-2 and TIMP-2 protein levels in cervical lesions have a positive correlation. Conclusion The expression of Ezrin, MMP-2 and TIMP-2 in cervical squamous cell carcinoma and adenocarcinoma is higher than that in control group. Ezrin and MMP-2 are highly expressed in cervical cancer with lymph node metastasis, while TIMP-2 is low expression, suggesting that they play an important role in the invasion and metastasis of cervical cancer. Ezrin, MMP-2 and TIMP-2 protein levels in cervical lesions have a positive correlation, which may play a synergistic role in the invasion and metastasis of cervical cancer.