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目的:探讨远程缺血预处理后,急性脑卒中24 h,72 h时脾脏组织Treg cells水平的表达变化,阐述远程缺血预处理对Treg cells的调节及其在急性脑卒中缺血期的保护作用。方法:60只昆明小鼠随机分为3组,sham组、MCAO组、远程缺血预处理+MCAO组(n=20只/组),建立小鼠MCAO模型,缺血60分再灌注后在24 h,72 h时进行神经功能缺陷评分(NDS),随后处死小鼠行氯化三苯基四氮唑(TTC)脑片染色,观察脑梗死容积变化。运用流式细胞仪检测脾脏组织Treg cells的表达。ELISA检测脑组织中炎症因子IFN-γ的表达。结果:在72 h时远程缺血预处理组与对照组相比,脑梗死容积减少,神经障碍减轻,脾脏Treg cells的表达上升,脑组织炎症因子IFN-γ的表达下降,P<0.05,而24 h Treg cells的表达水平并未发生明显变化,P>0.05。结论:在脑卒中急性期,远程缺血预处理通过升高Treg cells的表达参与脑保护作用。
OBJECTIVE: To investigate the expression of Treg cells in spleen at 24 and 72 h after acute ischemic preconditioning, and to elucidate the regulatory effect of remote ischemic preconditioning on Treg cells and their protection during acute ischemic stroke effect. Methods: Sixty Kunming mice were randomly divided into 3 groups: sham group, MCAO group and long-distance ischemic preconditioning + MCAO group (n = 20 / group). MCAO mice were established. Neurological deficit scores (NDS) were measured at 24 h and 72 h after transfection. The mice were sacrificed and stained with triphenyltetrazolium chloride (TTC). The volume of cerebral infarction was observed. The expression of Treg cells in spleen was detected by flow cytometry. ELISA was used to detect the expression of IFN-γ in brain tissue. Results: Compared with the control group, the ischemic preconditioning group at 72 h reduced the volume of cerebral infarction, reduced the neurological disorders, increased the expression of Treg cells in the spleen, and decreased the expression of IFN-γ in the brain tissue (P <0.05) 24 h Treg cells expression levels did not change significantly, P> 0.05. Conclusion: In the acute phase of stroke, remote ischemic preconditioning may play a role in cerebral protection by increasing the expression of Treg cells.