作者用生物素标记的探针检测常规骨髓活检切片(用三氯醋酸或EDTA脱钙),经杂交后,用链卵蛋白/生物素标记碱性磷酸酶及坚牢红显色.在14例可疑骨髓瘤的浆细胞有12例只表达轻链,其中9例最后肯定诊断为骨髓瘤.作者认为原位杂交是特异、安全和快速的技术,它可用于固定的或新鲜的标本.由于死亡或损伤的 The authors used biotin-labeled probes to detect routine bone marrow biopsies (decalcified with trichloroacetic acid or EDTA). After hybridization, they were stained with streptavidin/biotin to label alkaline phosphatase and fasting red. In 14 cases. Only 12 cases of suspicious myeloma plasma cells expressed only the light chain, of which 9 cases were definitely diagnosed as myeloma. The authors believe that in situ hybridization is a specific, safe, and rapid technique that can be used for fixed or fresh specimens. Because of death Or damaged