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目的:探讨猫爪草多糖(polysaccharide of Radix Ranunculi Ternati,PRT)对小鼠免疫功能的调节及抗氧化活性研究。方法:用MTT法检测PRT对小鼠胸腺细胞、脾脏淋巴细胞和腹腔巨噬细胞增殖活性的影响;用中性红比色法检测PRT对小鼠腹腔巨噬细胞吞噬功能的影响;采用普鲁士兰法测定PRT的还原能力;水杨酸捕捉法测定PRT对.OH的清除能力;邻苯三酚自氧化法测定PRT对O2.-的清除能力。结果:25～400 mg.L-1PRT呈剂量依赖式增强小鼠胸腺细胞和脾脏淋巴细胞增殖功能以及巨噬细胞的吞噬功能;PRT在200 mg.L-1时,对巨噬细胞增殖作用最强;在不同浓度梯度,PRT均表现出一定的还原能力;PRT在8 g.L-1时,接近同浓度Vit C对.OH自由基的清除作用;PRT在800 mg.L-1时,对O2.-的清除率达到95.39%。结论:PRT能增强小鼠胸腺细胞、脾脏淋巴细胞和腹腔巨噬细胞增殖能力,且对巨噬细胞有一最佳作用浓度;PRT能增强巨噬细胞的吞噬功能,并呈剂量-效应关系;PRT具有一定的还原能力,具有较强的清除.OH和O.2-的能力。
Objective: To investigate the regulation of immune function and the anti-oxidative activity of PRT in mice. Methods: The effects of PRT on the proliferation of thymocytes, spleen lymphocytes and peritoneal macrophages in mice were detected by MTT assay. The effect of PRT on the phagocytosis of peritoneal macrophages in mice was detected by neutral red colorimetry. Prussian blue Method to determine the reduction ability of PRT; salicylic acid capture method to determine the ability of PRT to scavenge OH; and pyrogallol auto-oxidation method to determine the ability of PRT to scavenge O2.-. Results: 25 ~ 400 mg.L-1 PRT increased the proliferation of thymocytes and spleen lymphocytes and the phagocytosis of macrophages in a dose-dependent manner. When PRT was 200 mg.L-1, the proliferation of macrophages was the most PRT showed a certain reduction ability under different concentration gradients. When PRT was at 8 gL-1, it was close to the scavenging effect of Vit C on the free radical. When PRT was 800 mg.L-1, .- The clearance rate reached 95.39%. PRT can enhance the phagocytic function of macrophages and have a dose-effect relationship; PRT can enhance the proliferation of mouse thymocytes, spleen lymphocytes and peritoneal macrophages, and have an optimal concentration of macrophages; PRT Has a certain ability to restore, with a strong ability to clear. OH and O.2-.