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目的研究尾静脉或蛛网膜下腔注射神经激肽(neurokinin,NK)-1受体拮抗剂GR82334对强电流刺激大鼠隐神经(saphenous nerve,SN)增强扣带回前部(anterior cingulate gyrus,ACG)Fos蛋白表达的影响。方法应用免疫组化技术进行实验研究。结果强电流刺激大鼠SN引起ACG Fos蛋白表达显著增强;尾静脉或蛛网膜下腔注射GR82334拮抗了强电流刺激大鼠SN引起的ACG Fos蛋白表达的显著增强。然而,蛛网膜下腔注射GR82334并没有完全拮抗强电流刺激大鼠SN引起的ACG Fos蛋白表达的显著增强。结论大鼠SN传导的伤害性信息能够到达ACG,激活c-fos基因表达;外周NK-1受体与中枢NK-1受体参与大鼠SN传入信息引起的ACG Fos蛋白表达增强的过程,但是,还存在其他递质和受体参与的大鼠SN信息传入的其它中枢通路引起ACG Fos蛋白表达的显著增强。
Objective To investigate the effect of GR82334, a neurokinin (NK) -1 receptor antagonist, injected into the tail vein or subarachnoid space on the anterior cingulate gyrus ACG) Fos protein expression. Methods Immunohistochemistry was used to carry out experimental study. Results Strong current stimulation of SN induced a significant increase of ACG Fos protein expression in rats. The injection of GR82334 into the tail vein or subarachnoid space significantly inhibited the SNP-induced increase of ACG Fos protein expression in rats. However, subarachnoid injection of GR82334 did not completely antagonize the significant enhancement of ACG Fos protein expression induced by high current stimulation of rat SN. CONCLUSIONS: The nociceptive information of SN in rats can reach ACG and activate the expression of c-fos gene. The process of peripheral blood NK-1 receptor and central NK-1 receptor involved in the expression of ACG Fos protein induced by SN in rat SNP, However, there are other central pathways into which other SNPs in the transmitter and receptor are involved in introducing a significant increase in ACG Fos protein expression.