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阐明康乐霉素Ｃ（Ｋａｎ）对脾细胞增殖和Ｔ－细胞亚型的作用。方法：氚掺入法或噻唑蓝（ＭＴＴ）比色法测定细胞增殖；用荧光激活细胞分选仪（ＦＡＣＳ）测定细胞亚型；曲利苯蓝排斥法测定细胞存活率．结果： Ｋａｎ ８， ４０， ８０和 ４００ ｎｍｏｌ· Ｌ－１，除抑制丝裂原（Ｃｏｎ Ａ， ＰＨＡ和 ＴＰＡ＋ＩＭ）和同种异型抗原刺激的小鼠脾细胞增殖外；与Ｃｉｃ不同，抑制ＬＰＳ （１０ｍｇ·Ｌ－１）刺激的脾细胞增殖；使Ｌ３Ｔ４＋／Ｌｙｔ２＋ Ｔ－细胞亚型比值倒置； Ｋａｎ于 Ｃｏｎ Ａ（ ５ｍｇ· Ｌ－１）刺激后 ２４ ｈ内加入，仍抑制脾细胞增殖． Ｋａｎ Ｂ－４００ ｎｍｏｌ· Ｌ－１不影响脾细胞存活率。结论：与Ｃｉｃ的作用方式不同，Ｋａｎ抑制Ｔ－和Ｂ－细胞活化的早期和晚期时相，抑制细胞增殖，对Ｔｎ－细胞有选择性。
The effect of kanamycin C (Kan) on splenocyte proliferation and T-cell subtypes was elucidated. METHODS: Cell proliferation was measured by tritium incorporation or MTT assay. Cell subtypes were determined by fluorescence activated cell sorter (FACS) and cell viability was determined by triptorelin blue exclusion. RESULTS: Kan 8, 40, 80 and 400 nmol · L-1 inhibited the proliferation of splenocytes stimulated by alloantigen except splenocytes (Con A, PHA and TPA + IM) The ratio of L3T4 + / Lyt2 + T-cell subsets was inverted. Kan was added within 24 h after stimulation with Con A (5 mg · L-1) and still inhibited the proliferation of spleen cells. Kan B-400 nmol · L-1 did not affect the survival rate of spleen cells. CONCLUSIONS: Unlike Cic, Kan inhibits the early and late phases of T- and B-cell activation, inhibits cell proliferation, and is selective for Tn-cells.