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目的研究夏枯草对淋巴瘤细胞(Raji细胞)生长的影响及可能的机制。方法参考临床常用剂量,采用50g/L夏枯草(夏枯草组)、50g/L夏枯草+20μmol/LJNK特异抑制剂SP600125(SP600125组)、50g/L夏枯草+1μmol/LAkt特异抑制剂Wortmannin(Wortmannin组)处理Raji细胞,以同体积生理盐水作为对照组,应用MTT法检测各组的细胞增殖率,蛋白免疫印迹法检测各组的JNK和Akt磷酸化水平。结果夏枯草组细胞增殖率低于对照组(P<0.05),SP600125组细胞增殖率比夏枯草组增高(P<0.05),Wortmannin组细胞增殖率比夏枯草组降低(P<0.05);JNK磷酸化水平在夏枯草组中明显升高(P<0.05),SP600125可以抑制其磷酸化(P<0.05),Wortmannin不能抑制其磷酸化(P>0.05);Akt磷酸化水平在夏枯草组中降低(P<0.05),SP60015及Wortmannin均可抑制其磷酸化(P<0.05)。结论夏枯草可以明显抑制Raji细胞的生长,这种抑制作用可能是通过激活JNK信号转导通路,抑制Akt通路激活实现的。
Objective To study the effect of Prunella vulgaris on the growth of lymphoma cells (Raji cells) and its possible mechanism. Methods The clinically used doses were referenced using 50 g/L Prunella vulgaris (Prunella vulgaris group), 50 g/L Prunella vulgaris + 20 μmol/L JNK specific inhibitor SP600125 (SP600125 group), 50 g/L Prunella vulgaris +1 μmol/LAkt specific inhibitor Wortmannin ( The Wortmannin group was treated with Raji cells. The same volume of normal saline was used as a control group. The proliferation rate of each group was detected by MTT assay. The phosphorylation levels of JNK and Akt in each group were detected by Western blotting. Results The cell proliferation rate of Prunella vulgaris was lower than that of the control group (P<0.05). The cell proliferation rate of SP600125 group was higher than that of Prunella vulgaris group (P<0.05). The cell proliferation rate of Wortmannin group was lower than that of Prunella vulgaris group (P<0.05); JNK Phosphorylation was significantly increased in the Prunella vulgaris group (P<0.05), SP600125 could inhibit its phosphorylation (P<0.05), Wortmannin could not inhibit its phosphorylation (P>0.05), and Akt phosphorylation level was in the Prunella group. Decreased (P<0.05), SP60015 and Wortmannin inhibited their phosphorylation (P<0.05). Conclusion Prunella vulgaris can obviously inhibit the growth of Raji cells. This inhibition may be achieved by activating JNK signal transduction pathway and inhibiting activation of Akt pathway.