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目的:建立HPLC同时测定楤木不同部位绿原酸、咖啡酸、3,5-二咖啡酰奎宁酸及4,5-二咖啡酰奎宁酸含量的方法。方法:色谱柱:Dikma Kromasil C18柱(250 mm×4.6mm,5μm);流动相:乙腈-0.1%磷酸水溶液,梯度洗脱;测定波长:325 nm。结果:绿原酸、咖啡酸、3,5-二咖啡酰奎宁酸及4,5-二咖啡酰奎宁酸的线性范围分别为0.101～5.040μg(r=0.999 8),0.003～0.128μg(r=0.999 6),0.078～3.900(r=0.999 8),0.014～0.715(r=0.999 7)。平均回收率分别为99.10%(RSD为0.96%),98.26%(RSD为1.38%),99.25%(RSD为0.83%),98.53%(RSD为1.22%)。结论:该方法简便快速、具有良好的重复性和回收率,可作为楤木中这4种成分的定量分析方法。
OBJECTIVE: To establish a method for the simultaneous determination of chlorogenic acid, caffeic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in different parts of Alnus cremastogyne by HPLC. Methods: Column: Dikma Kromasil C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-0.1% phosphoric acid aqueous solution, gradient elution; Results: The linear ranges of chlorogenic acid, caffeic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid were0.01 ~ 5.040μg (r = 0.999 8), 0.003 ~ 0.128μg (r = 0.999 6), 0.078 ~ 3.900 (r = 0.999 8) and 0.014 ~ 0.715 (r = 0.999 7). The average recoveries were 99.10% (RSD 0.96%), 98.26% (RSD 1.38%), 99.25% (RSD 0.83%) and 98.53% (RSD 1.22%), respectively. Conclusion: The method is simple and rapid with good repeatability and recovery, which can be used as a quantitative analysis method for the four components in Alnus creitis.