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目的:研究酸性磷酸酶(acid phosphatase,ACP)在子宫颈癌组织中的表达情况及其临床意义。方法:采用RT-PCR、Western blot和免疫组化方法分别检测30例子宫颈鳞状细胞癌和30例正常宫颈组织中ACP的表达情况。结果:RT-PCR结果显示,子宫颈癌组织中ACP mRNA表达水平明显高于正常宫颈组织,但差异无统计学意义(P>0.05);Western blot结果显示,子宫颈癌组织中ACP蛋白的表达水平明显高于正常宫颈组织,差异有统计学意义(P<0.05);免疫组化结果显示,ACP定位于细胞浆,同时也提示子宫颈癌组织与正常宫颈组织比较,ACP表达明显升高(P<0.05)。结论:ACP在子宫颈癌组织中的表达明显升高,可以作为子宫颈癌的一项新的辅助诊断指标。
Objective: To study the expression of acid phosphatase (ACP) in cervical cancer and its clinical significance. Methods: The expression of ACP in 30 cases of cervical squamous cell carcinoma and 30 cases of normal cervical tissue were detected by RT-PCR, Western blot and immunohistochemistry respectively. Results: RT-PCR results showed that the expression of ACP mRNA in cervical cancer tissues was significantly higher than that in normal cervical tissues, but the difference was not statistically significant (P> 0.05). Western blot results showed that the expression of ACP in cervical cancer tissues (P <0.05). The results of immunohistochemistry showed that ACP was located in the cytoplasm, which also suggested that the expression of ACP in cervical cancer was significantly higher than that in normal cervix P <0.05). Conclusion: The expression of ACP in cervical cancer tissues is obviously increased, which may serve as a new diagnostic indicator of cervical cancer.