论文部分内容阅读
为研究新疆葡萄中沙地葡萄茎痘伴随病毒(Grapevine rupestris stem pitting associated virus,GRSPa V)、葡萄斑点病毒(Grapevine fleck virus,GFk V)及葡萄病毒A(Grapevine virus A,GVA)的发生情况和新疆分离株系统进化关系,分别克隆3种病毒新疆分离株部分基因区域,应用RT-PCR对新疆64份葡萄样品中上述3种病毒进行检测,并进行系统进化分析。结果显示,GRSPa V、GFk V和GVA的检出率分别为31.3%、62.5%和25.0%。新疆GRSPa V分离株(KJ801847)与美国GRSPa V分离株(AY368590)同源性达96.59%;新疆GFk V分离株(KJ801846)与日本GFk V分离株(AB222861)及中国辽宁GFk V分离株(JF927942)的同源性分别为91.70%和91.03%;新疆GVA分离株(KJ801845)与波兰GVA分离株(JN860997)同源性为93.88%,与中国四川GVA分离株(HQ671655)及辽宁GVA分离株(FJ445220)的同源性分别为92.92%和89.53%。表明3种葡萄病毒在新疆发生比较普遍,且新疆分离株与国内其它地方的分离株存在较大差异。
In order to study the occurrence of Grapevine rupestris stem pitting associated virus (GRSPa V), Gravevine fleck virus (GFk V) and Grapevine virus A (GVA) in grapes in Xinjiang, The phylogenetic relationships among Xinjiang isolates were established. The partial regions of three strains of Xinjiang isolates were cloned. The three viruses in 64 grape samples from Xinjiang were detected by RT-PCR and phylogenetic analysis was performed. The results showed that the detection rates of GRSPa V, GFk V and GVA were 31.3%, 62.5% and 25.0% respectively. The homology between Xinjiang GRSPa V isolate (KJ801847) and the American GRSPa V isolate (AY368590) was 96.59%. The GFK V isolate (KJ801846) and the Japanese GFk V isolate (AB222861) from Xinjiang and the GFk V isolate from Liaoning (JF927942 ) Were 91.70% and 91.03%, respectively. The homology between Xinjiang GVA isolate (KJ801845) and Polish GVA isolate (JN860997) was 93.88%, which was highly homologous with GVA isolates from China (HQ671655) and Liaoning GVA isolates FJ445220) were 92.92% and 89.53%, respectively. The results showed that three kinds of grapevine viruses were common in Xinjiang, and there were big differences between Xinjiang isolates and other isolates in China.