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目的探讨hMTH1Val83Met、hOGG1Ser326Cys和hMYHHis335Gln基因多态性与慢性苯中毒发病风险的关系。方法采用病例对照设计,以152名苯中毒工人为病例组,152名接触苯而没有中毒表现的工人为对照组。应用聚合酶链反应-限制性片断长度多态性分析技术(PCRRFLP)检测hMTH1c.83、hOGG1c.326和hMYHc.335位点的多态性。结果携带hMTH1c.83ValMet+MetMet和hOGG1c.326CysCys基因型的个体发生慢性苯中毒的风险性分别是携带hMTH1c.83ValVal和hOGG1c.326SerCys+SerSer基因型个体的2.51倍(ORadj=2.51,95%CI:1.14～5.49,P=0.02)和2.49倍(ORadj=2.49,95%CI:1.52～4.07,P<0.01);相比于同时携带hOGG1c.326CysCys和hMYHc.335HisHis基因型的个体,同时携带hOGG1c.326SerCys+SerSer和hMYHc.335HisGln+GlnGln两种基因型的个体有较低的慢性苯中毒发病风险(ORadj=0.33,95%CI=0.15～0.72,P=0.01);在经常吸烟的人群中,携带hMYHc.335HisGln+GlnGln基因型的个体慢性苯中毒的发病风险较携带hMYHc.335HisHis基因型的个体降低(ORadj=0.15,95%CI:0.03～0.68,P=0.01)。结论hMTH1Val83Met和hOGG1Ser326Cys多态可能与个体慢性苯中毒的发病风险改变有关,而hOGG1Ser326Cys和hMYHHis335Gln基因多态之间可能存在潜在的联合作用。
Objective To investigate the relationship between hMTH1Val83Met, hOGG1Ser326Cys and hMYHHis335Gln gene polymorphisms and the risk of chronic benzene poisoning. Methods A case-control study was designed. Among 152 benzene-poisoned workers, 152 were benzene-exposed workers without poisoning. Polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) was used to detect the polymorphisms of hMTH1c.83, hOGG1c.326 and hMYHc.335. Results The risk of chronic benzene poisoning in individuals carrying the hMTH1c.83ValMet + MetMet and hOGG1c.326CysCys genotypes was 2.51-fold (ORadj = 2.51, 95% CI: 1.14 (ORadj = 2.49, 95% CI: 1.52-4.07, P <0.01). Compared with individuals carrying both hOGG1c.326CysCys and hMYHc.335HisHis genotypes, hOGG1c.326SerCys + SerSer and hMYHc.335HisGln + GlnGln genotypes had lower risk of chronic benzene poisoning (ORadj = 0.33, 95% CI = 0.15-0.72, P = 0.01); in those who regularly smoked, those who carried hMYHc Individuals with the .335HisGln + GlnGln genotype had a lower risk of developing chronic benzene poisoning than those with the hMYHc.335HisHis genotype (ORadj = 0.15, 95% CI: 0.03-0.68, P = .01). Conclusion The hMTH1Val83Met and hOGG1Ser326Cys polymorphisms may be related to the risk of chronic benzene poisoning. There may be a potential synergistic effect between hOGG1Ser326Cys and hMYHHis335Gln polymorphisms.