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目的 :建立牛肾上腺毛细血管内皮细胞的长期培养的方法。 方法 :无菌取出新生牛肾上腺进行长期培养 ,观察其生物学特性。 结果 :牛肾上腺毛细血管内皮细胞在加入肿瘤条件培养基及碱性成纤维细胞生长因子 (b FGF)培养液中 ,能够长期培养至 31代 ,不同代次细胞 r-谷氨酰胺转酶 (GGT) ,碱性磷酸酶 (AKP)等生化指标稳定。 结论 :在含肿瘤条件培养基及 b FGF培养液中能够长期培养牛肾上腺毛细血管内皮细胞。
Objective: To establish a method of long-term culture of bovine adrenal capillary endothelial cells. Methods: Neonatal bovine adrenal glands were removed aseptically for long-term culture to observe its biological characteristics. Results: Bovine adrenal capillary endothelial cells were cultured in the condition of tumor conditioned medium and basic fibroblast growth factor (bFGF) for a long time, and could be cultured for 31 generations. The expression of transglutaminase (GGT) ), Alkaline phosphatase (AKP) and other biochemical indicators of stability. CONCLUSION: Bovine adrenal capillary endothelial cells can be cultured for a long time in tumor-conditioned medium and bFGF medium.