论文部分内容阅读
目的:研究苦参碱(Mat)对脂多糖(lipopolysaccharides,LPS)诱导的D-氨基半乳糖(D-GalN)致敏小鼠致死性肝炎以及腹腔巨噬细胞(PM)释放肿瘤坏死因子(TNF)的影响。方法:小鼠ip Mat10,50mg·kg~(-1),bid×3d,然后ip LPS(1μg·kg~(-1))和D-GalN(800mg·kg~(-1)),通过病理组织学观察及测定血清丙氨酸转氨酶(ALT)活性来评估肝损伤。小鼠PM培养上清中的TNF活性以杀伤L929细胞的结晶紫染色法测定。结果:Mat降低了LPS/D-GalN引起的血清ALT活性升高及小鼠对LPS/D-GalN致死毒性的敏感性并抑制LPS诱导的小鼠PM释放TNF。结论:Mat防治LPS/D-GalN引起的致死性肝炎,并抑制LPS诱导的TNF释放。
OBJECTIVE: To study matrine (Mat) induced fatal hepatitis in D-GalN-sensitized mice induced by lipopolysaccharides (LPS) and release of tumor necrosis factor (TNF) from peritoneal macrophages (PM). )Impact. METHODS: Mice ip Mat10, 50 mg·kg -1, bid × 3 d, then ip LPS (1 μg·kg -1) and D-GalN (800 mg·kg -1), passed the pathology Histological observation and determination of serum alanine aminotransferase (ALT) activity was used to assess liver injury. The TNF activity in mouse PM culture supernatants was determined by crystal violet staining to kill L929 cells. RESULTS: Mat reduced the serum ALT activity induced by LPS/D-GalN and the sensitivity of mice to LPS/D-GalN lethal toxicity and inhibited LPS-induced PM release from mice. Conclusion: Mat prevents lethal hepatitis caused by LPS/D-GalN and inhibits LPS-induced TNF release.