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目的：检测黑素瘤抑制蛋白（ＭＩＡ／ＣＤ－ＲＡＰ）在小鼠胚胎乳腺组织中的表达。方法：剖腹分离出１０．５～１６．５ ｄ 的ＭＩＡ／ＣＤ－ＲＡＰ启动子－半乳糖苷酶（２．２ｌａｃＺ）转基因小鼠胚胎，利用Ｘ－ｇａｌ全胚胎染色观察转基因的组织和细胞染色，并以免疫组织化学染色检测内源性ＭＩＡ／ＣＤ－ＲＡＰ蛋白在胚胎乳腺组织中的表达。结果：ＭＩＡ／ＣＤ－ＲＡＰ在孕期１１．５ ｄ 的小鼠胚胎乳腺上皮细胞中开始表达，至１３．５ ｄ 表达明显增强，而后逐渐减弱，至１６．５ ｄ 消失。结论：ＭＩＡ／ＣＤ－ＲＡＰ可能在乳腺的早期发育和乳腺癌发生中起重要作用。
Objective: To detect the expression of melanoma inhibitor protein (MIA / CD-RAP) in mouse embryonic breast tissue. METHODS: MIA / CD-RAP promoter-galactosidase (2.2lacZ) transgenic mouse embryos were isolated from 10.5-16.5 d by Caesarean section. Tissue and cell staining of transgenic tissues were observed by X-gal whole embryo staining , And the expression of endogenous MIA / CD-RAP protein in embryonic breast tissue was detected by immunohistochemical staining. Results: MIA / CD-RAP began to express in mouse embryonic mammary epithelial cells at 11.5 days of gestation. The expression of MIA / CD-RAP was significantly enhanced at 13.5 days and then gradually weakened and disappeared after 16.5 days. Conclusion: MIA / CD-RAP may play an important role in the early development of mammary gland and in the development of breast cancer.