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目的 探讨新生儿吸入性肺炎和肺出血与单纯疱疹病毒(herpes simplex virus,HSV)感染的关系。方法 采用实时荧光定量聚合酶链反应的方法(fluorescenee quantitative polymerase chain reaction,FQ-PCR)检测26例因吸入性肺炎和19例因肺出血死亡的新生儿肺组织中HSV-DNV。结果 45例肺组织中,共检出HSV-DNA 19例,总检出率为42.2％(19/45),其中26例吸入性肺炎肺组织中检出10例,检出率为38.5％(10/26),19例肺出血肺组织中检出9例,检出率为47.4％(10/19);吸入性肺炎与肺出血组的HSV-DNA定量值分别为(3.65±7.24)×10~5与(8.26±2.27)×10~6,2者比较差异无显著性(P>0.05);HSV-DNA定量值大于1.0×10~5基因拷贝/毫克与小于1.0×10~5基因拷贝/毫克比较,前者临床症状重,死亡时间早。结论 在新生儿吸入性肺炎和肺出血致死因素中,应注意HSV感染的作用。对HSV定量值大于1.0×10~5基因拷贝/毫克的患儿,加强抗病毒的治疗可能是必要的。
Objective To investigate the relationship between neonatal aspiration pneumonia and pulmonary hemorrhage and herpes simplex virus (HSV) infection. Methods Fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect HSV-DNV in lung tissue of 26 infants with aspiration pneumonia and 19 died of pulmonary hemorrhage. Results Of the 45 cases of lung tissue, 19 cases were detected HSV-DNA, the total detection rate was 42.2% (19/45), of which 26 cases of aspiration pneumonia were detected in 10 cases, the detection rate was 38.5% 9 cases were detected in 19 cases of pulmonary hemorrhage and the detection rate was 47.4% (10/19). The quantitative values of HSV-DNA in aspiration pneumonia and pulmonary hemorrhage were (3.65 ± 7.24) × There was no significant difference between 10 ~ 5 and (8.26 ± 2.27) × 10 ~ 6 (P> 0.05). The quantitative value of HSV-DNA was greater than 1.0 × 10 ~ 5 copies / mg and less than 1.0 × 10 ~ 5 Copy / mg comparison, the former clinical symptoms, death early. Conclusion In the neonatal aspiration pneumonia and pulmonary hemorrhage lethal factors, should pay attention to the role of HSV infection. For children with HSV quantitation greater than 1.0 × 10 5 copies / mg, it may be necessary to augment antiviral treatment.