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用蒸馏水振荡浸提土壤确可从滤液中检测出游离脲酶活性,但其量很少,不到土壤总酶活性的1%,且土壤层次越深,土壤游离脲酶有增加的趋势.塑料管柱模拟实验表明,这些游离酶仅有少量(7～17%)可随水移动.用黄豆粉作为外源游离脲酶加入脲酶活性很低的底层土进行对比实验表明,游离脲酶对尿素的水解速率远大于活性相当的被土壤所保持的脲酶对尿素的水解速率.可以推断,土壤游离脲酶对加入土壤的尿素的水解作用可能更直接些,但尚需进一步研究.
However, the amount of free urease activity was detected by the filtrate from the leaching soil with distilled water, but its amount was very little, less than 1% of the total soil enzyme activity, and the deeper the soil layer, the more the free urease activity was. Simulated experiments showed that only a small amount of these free enzymes (7-17%) could move with the water.Compared with the soils with low urease activity, soy bean flour was used as the exogenous free urease to show that the hydrolysis rate of urea by the free urease was far greater The rate of urea hydrolysis by urease retained by the soil is comparable with that of the soil urease activity, and it can be inferred that the hydrolysis of soil-bound urease may be more direct, but further study is needed.