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目的:探讨清开灵注射液(QKL)的抗血栓作用及其机制。方法:将SD大鼠随机分为对照组、模型组、QKL 2.5,5.0,10.0 mL.kg-1组。QKL各剂量组均ip给药,每天1次×4 d。除正常对照组外,其余各组均于末次给予QKL前16 hip角叉菜胶(Ca)5 mg/只(0.5 mL)。于末次给予QKL后30 min,除了正常对照组iv生理盐水1 mL.kg-1外,其余各组均iv给予内毒素(LPS)50μg.kg-1(1 mL.kg-1),以注射LPS时作为造模0 h。于造模后2 h取血测定血浆TNF-α,IL-6含量和白细胞黏附分子CD11b/CD18表达;4,24 h测定外周血白细胞和血小板数量和测量尾部血栓长度(cm);120 h测定血小板聚集率。结果:清开灵注射液可明显抑制血栓形成,缩短尾部血栓长度;明显抑制体内炎性因子TNF-α和IL-6产生,降低白细胞黏附分子CD11b/CD18的表达水平;减轻由于血栓形成而导致的血小板消耗;降低血小板聚集率。结论:清开灵注射液的抗血栓作用与其抑制白细胞的激活和黏附,降低炎症反应和炎性因子产生,抑制血小板聚集等有关,这些作用机制与其清热解毒功效一致。
Objective: To investigate the anti-thrombotic effect of Qing Kai Ling Injection (QKL) and its mechanism. Methods: SD rats were randomly divided into control group, model group, QKL 2.5, 5.0, 10.0 mL.kg-1 group. Each QKL dose group was administrated ip once a day for 4 days. Except for the normal control group, the rest of the groups were given QKL before the 16 hip carrageenan (Ca) 5 mg/body (0.5 mL). At 30 min after the last administration of QKL, except for the normal control group, iv normal saline (1 mL.kg-1), all other groups received iv endotoxin (LPS) of 50 μg.kg-1 (1 mL.kg-1) for injection. LPS is used as model 0h. At 2 hours after model establishment, blood was taken to measure plasma TNF-α and IL-6 levels and CD11b/CD18 expression; leukocyte and platelet counts were measured at 4 and 24 h, and tail thrombus length (cm) was measured; measured at 120 h. Platelet aggregation rate. Results: Qingkailing injection can significantly inhibit thrombosis, shorten the length of tail thrombus; significantly inhibit the production of inflammatory cytokines TNF-α and IL-6 in the body, and reduce the expression level of leukocyte adhesion molecule CD11b/CD18; and reduce the formation of thrombosis. Platelet consumption; reduced platelet aggregation rate. Conclusion: The antithrombotic effect of Qingkailing injection is related to its inhibition of leukocyte activation and adhesion, decrease of inflammatory response and production of inflammatory factors, inhibition of platelet aggregation, etc. These mechanisms of action are consistent with its detoxification efficacy.