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目的 研究和探索hTFRT反义核酸对淋巴白血病细胞端粒酶活性的影响。方法 用PCR ELISA试剂合测定端粒酶的活性;免疫组织化学和流式细胞仪技术测定hTERT蛋白的含量;用RT-PCR和电泳技术测定hTERT mRNA水平。结果 实验结果显示,10μmol/L AS PS-ODN作用72 h以后,hTERT mRNA和蛋白的水平显著下降,端粒酶的活性明显受到抑制。结论 hTERT反义核酸是淋巴白血病细胞端粒酶活性的良好的抑制剂。
Objective To study and explore the effect of hTFRT antisense nucleic acid on telomerase activity in lymphoid leukemia cells. Methods The activity of telomerase was determined by PCR ELISA. The content of hTERT protein was determined by immunohistochemistry and flow cytometry. The hTERT mRNA level was determined by RT-PCR and electrophoresis. Results The results showed that after treated with 10 μmol / L AS PS-ODN for 72 h, the mRNA and protein levels of hTERT significantly decreased and the activity of telomerase was significantly inhibited. Conclusion hTERT antisense nucleic acid is a good inhibitor of telomerase activity in lymphoid leukemia cells.