目的:研究的目的是阐明在ATP缺乏的免近端小管(PT)中,自微绒毛内肌动蛋白细胞骨架上崩解的肌动蛋白是否与非肌动蛋白结合。方法:首先用DTSSP交联存在于ATP缺乏的PT不溶组分中肌动蛋白和非肌动蛋白结合的方法,交联复合物进一步用二维电泳及免疫印迹法分析。结果:分析被交联的肌动蛋白复合物发现肌动蛋白与非肌动蛋白结合,而并非与绒毛蛋白结合。结论:本结果首次提示:在ATP缺乏的免PT中,源于肌动蛋白细胞骨架的部分肌动蛋白是与非肌动蛋白相结合,而不与绒毛蛋白直接结合或以疏水性共聚体与核共存的。 PURPOSE: The aim of the study was to elucidate whether actin disrupted in the actin cytoskeleton of microvilli binds to non-actin in the ATP-deficient, proximal tube (PT). Methods: Firstly, DTSSP was used to cross-link the actin and non-actin in PT-insoluble ATP-deficient fractions. The cross-linked complexes were further analyzed by two-dimensional electrophoresis and Western blotting. Results: Analysis of the crosslinked actin complex revealed that actin binds to non-actin and not to villin. CONCLUSIONS: This result, for the first time, suggests that in ATP-deficient, PT-free, part of the actin derived from the actin cytoskeleton is bound to non-actin rather than directly to villous proteins or to hydrophobic inter- Nuclear coexistence.