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本文应用差文库（ｓｕｂｔｒａｃｔｉｖｅｌｉｂｒａｒｙ）富集酒精性肝硬化的高表达基因。从酒精性肝硬化大鼠建立单方向ｃＤＮＡ基因文库，并提取单键ｃＤＮＡ。以４０倍的正常鼠肝ｍＲＮＡ标记生物素后，对肝硬化鼠肝的单链ｃＤＮＡ进行差杂交，滤掉表达相似序列。共得约９０个克隆，其中１０个克隆分析了约５００个碱基对。一个克隆的ＤＮＡ序列分析了８００个碱基对。查Ｇｅｎｅｂａｎｋ，证实为一新的基因。Ｎｏｒｔｈｅｒｎ印迹分析显示肝硬化时，其ｍＲＮＡ的表达增加数倍。
In this paper, we use subtractivelibrary to enrich the highly expressed genes of alcoholic cirrhosis. One-way cDNA gene library was constructed from alcoholic cirrhotic rats and single-bond cDNA was extracted. After 40-fold normal mouse liver mRNA was labeled with biotin, the single-stranded cDNA of cirrhotic liver was poorly hybridized and the similar sequence was filtered out. A total of about 90 clones were obtained, of which 10 clones analyzed about 500 base pairs. One cloned DNA sequence analyzed 800 base pairs. Check Genebank, confirmed as a new gene. Northern blot analysis showed that the expression of mRNA increased several fold when cirrhotic.