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木瓜酶广泛用于血型血清学中协助不完全抗体的检测,但其活力测定方法不易规范化。现介绍一种可使木瓜酶测定标准化的方法,其受温度、pH、孵育时间影响极小。可消除不同工作者的差异。该法用特异性木瓜酶抑制剂E-64即L-反式环氧丁二酰-亮氨酰胺(4胍基)-丁烷测定木瓜酶活性。E-64与木瓜酶反应迅速,不可逆,能使酶活性完全受抑制,并与木瓜酶活性巯基等分子结合。在木瓜酶试
Papain is widely used in blood group serology to assist the detection of incomplete antibodies, but its activity determination method is not easy to standardize. A method for standardizing papain assay is presented, which is minimally affected by temperature, pH, and incubation time. Can eliminate the differences between different workers. Papaya enzyme activity was assayed using the specific papain enzyme inhibitor E-64, namely L-trans-epoxysuccinyl-leucinamide (4-guanidino) -butane. E-64 and papain rapid reaction, irreversible, can completely inhibit the activity of the enzyme, and papaya enzyme activity and other molecules mercapto binding. Try the papaya enzyme