目的　通过分析一起食物中毒剩余食品中所检出 2株非O1群霍乱弧菌 (血清型VBO12 )的鉴定结果 ,探讨非O1群霍乱弧菌的病原学特征。方法　细菌的分离和鉴定按照卫生部《霍乱防治手册》进行。结果　经过分离培养、生化反应、血清学鉴定、药物敏感试验 ,确定这次食物中毒剩余食品中所分离的 2株菌为非O1群霍乱弧菌 (血清型VBO12 )。结论　由非O1群霍乱弧菌引起的急性腹泻已经逐渐增多 ,它所引起的食物中毒也有报道。因此 ,今后检测部门必须高度重视腹泻病人中非O1群霍乱弧菌的检测工作 Objective To investigate the etiological characteristics of non-O1 group Vibrio cholerae by analyzing the identification results of two non-O1 group V. cholerae (serotype VBO12) detected in the remaining foodstuffs. Methods The isolation and identification of bacteria were carried out in accordance with the “Cholera Prevention and Control Manual” issued by the Ministry of Health. Results After isolation and culture, biochemical reaction, serological identification and drug susceptibility test, it was confirmed that the two strains isolated from the remaining food poisoning were Vibrio cholera non-O1 (serotype VBO12). Conclusions The acute diarrhea caused by Vibrio cholerae other than O1 group has gradually increased, and food poisoning caused by it has also been reported. Therefore, the detection department in the future must attach great importance to the detection of non-O1 Vibrio cholerae in diarrhea patients