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目的了解泰安市婴幼儿食品中阪崎肠杆菌的污染及耐药性和毒力状况,为婴幼儿食品安全管理及食源性疾病预警和临床用药提供科学依据。方法依据GB 4789—2010检测程序,连续4年(2011年-2014年)对泰安市4类婴幼儿食品(包括奶粉、米粉、营养饼干、面制品)中的阪崎肠杆菌进行分离培养及鉴定,采用CLSI推荐的微量肉汤稀释法检测药物敏感性,采用PCR技术检测毒力基因,采用血清凝集法进行血清分型。结果 2011年-2014年共检出7株阪崎肠杆菌,4类婴幼儿食品检出率差异无统计学意义(χ2=3.172,P=0.366)。7株阪崎肠杆菌人溶菌酶(hly)毒力基因检测全部阳性。血清分型结果 O1型5株,O6型2株。7株阪崎肠杆菌对头孢西丁的耐药率为100%,除四环素外,对测定的其他抗生素全部敏感。结论奶粉中阪崎肠杆菌的检出率较高,其血清型主要集中在O1型和O6型,全部携带hly毒力基因,对头孢西丁耐药情况较严重,对测定的其他抗生素敏感。
Objective To understand the contamination, drug resistance and virulence of Enterobacter sakazakii in infants and toddlers’ food in Taian and to provide a scientific basis for the early warning and clinical treatment of food safety in infants and young children. Methods According to the test procedure of GB 4789-2010, Enterobacter sakazakii in four types of infants and toddlers’ food (including milk powder, rice noodles, nutritional crackers, flour products) in Tai’an for four consecutive years (2011-2014) were isolated and cultured , The drug sensitivity was tested by CLSI recommended microdilution method, the virulence genes were detected by PCR, and the serotypes were detected by serum agglutination. Results A total of 7 strains of Enterobacter sakazakii were detected in 2011-2014. There was no significant difference in the detection rate of food among 4 types of infants (χ2 = 3.172, P = 0.366). Seven strains of Enterobacter sakazakii human lysozyme (hly) virulence genes were all positive. Serological typing results O1 type 5, O6 type 2 strains. Enterobacter sakazakii 7 strains of cefoxitin resistance was 100%, with the exception of tetracycline, all the other antibiotics tested sensitive. Conclusions The detection rate of Enterobacter sakazakii in milk powder is high. The serotypes are mainly O1 and O6, all carrying hly virulence genes, which are more resistant to cefoxitin resistance and sensitive to the other antibiotics tested.