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制备了高效价特异兔抗尿激酶血清,由此抗血清制备了抗尿激酶IgG.建立了ELISA和生物素-亲和素-ELISA(BA-ELISA)检测尿激酶方法.两种方法均具有良好的特异性.ELISA方法检测尿激酶的敏感性为0.1～0.06IU/100μl;BA-ELISA方法检测尿激酶的敏感性为0.01IU/100μl.以酶激尿标准品做参照物可进行定量测定.
Anti-urokinase IgG was prepared by antiserum with high valence-specific rabbit anti-urokinase serum, and ELISA and biotin-avidin-ELISA (BA-ELISA) were established to detect urokinase.All the methods were well The sensitivity of the ELISA method for the detection of urokinase was 0.1 to 0.06 IU / 100 μl, the sensitivity of the BA-ELISA method for detecting urokinase was 0.01 IU / 100 μl, and the enzymatic urine standard was used as a reference substance for quantitative determination.